Sequence analysis of exon 1 and intron 1 of growth hormone gene in six chicken genotypes raised in tropical environment

Alpha % Growth hormone gene Nigerian indigenous chicken SNPs KR nema Chicken growth (cGH) is a polypeptide secreted by the pituitary gland which responsible for several functions such as tissue and reproduction in chickens. This study was conducted to characterize six genotypes using exon 1 intron regions of cGH sequences. One hundred thirty-four (134) chickens comprising Normal feather (19), Naked neck (21), Frizzle (8), Arbor Acre (24), FUNAAB Alpha-1 (dihybrid) (31), FUNAAlpha-2 (trihybrid) (31) were used study. Blood samples collected from birds into EDTA bottles DNA extraction. The amplified published primers. product polymerase chain reaction subjected Sanger sequencing. DnaSP5 software determine diversity indices MEGA6 phylogenetic relationships among other Fifteen (15) identified none all genotypes, nine (9) occurred transitions while others transversions. allele frequency ranged 0.30 0.95 highest heterozygosity (0.66) observed mutation 410A>C genotype lowest at SNP 330C>T. Polymorphic Information Content (PIC) maximum with value 0.92. phylogeny tree revealed two clades where diverged. Intron clustered Alpha-1, together one sub-clades second clade. Network analysis major ancestor genotypes. concluded that polymorphic investigated, this can be candidate selection growth-related traits.